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How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define sample names in column A (≤ 25 characters)
Step 3: Define corresponding primer names in column B (≤ 25 characters; not necessary for primer sources "Standard Primer" or "Custom Primer". Optional for primer source "premixed")
Step 4: Copy/paste sample and primer names into the foreseen window
Step 5: Press the "Vertical Import" button if you want to import your date in a vertical manner (A1, B1, C1, etc.) or "Horizontal Import" in case you prefer a horizontal arrangement of your data on the plate (A1, A2, A3, etc.)

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define sample names in column A (≤ 17 characters)
Step 3: Define corresponding primer names in column B (≤ 17 characters; not necessary for primer sources "Standard Primer" or "Custom Primer". Optional for primer source "premixed")
Step 4: Copy/paste sample and primer names into the foreseen window
Step 5: Press the "Import" button

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define sample names in column A (≤ 17 characters)
Step 3: Define corresponding primer names in column B (≤ 17 characters; not necessary for primer sources "Standard Primer" or "Custom Primer". Optional for primer source "premixed")
Step 4: Copy/paste sample and primer names into the foreseen window
Step 5: Press the "Import" button

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Choose an oligo from your list of Favorites in the drop-down menu.
Step 2: Press "Add" to add the chosen oligo to your order.

Technical Datasheet and Different Types of Certificate of Analysis (CoA)

On our technical datasheet, you find information regarding your ordered oligos like oligo name, oligo ID, nucleotide sequence, modifications, length, synthesis scale, molecular weight, melting point, the amount in OD, nmol and µg and the information with how much water you need to dissolve the dried oligo to obtain a 100 µM solution.

The technical datasheet does not meet your requirements?
For an extra charge, we do offer different types of CoAs for a higher level of documentation. Our standard CoA (Type 1) contains the following information:

Further to the standard CoA are the following types of CoA's available:

Type Description
1 Certificate of Analysis (complete)
2 CoA without declaration of oligonucleotide stability
3 CoA without Maldi TOF
4 CoA without analytical HPLC
5 only Maldi TOF spectra
6 only HPLC

You need a customized CoA? Please get in contact with us.

How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define oligo names in column A (≤ 25 characters)
Step 3: Define corresponding oligo sequences in column B
Step 4: Click on "Copy/paste"
Step 5: Copy/paste the oligo data from Excel into the foreseen window and press OK

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
Microsynth Oligonucleotides
Please be aware that Microsynth’s oligonucleotides are not sold through CARLO ERBA Reagents S.r.l. Oligonucleotide orders will be invoiced through Microsynth to the billing address in your customer data.

Microsynth Oligonucleotidi
Si prega di notare che gli oligonucleotidi di Microsynth non sono venduti tramite CARLO ERBA Reagents S.r.l. Gli ordini di oligonucleotidi verranno fatturati tramite Microsynth all'indirizzo di fatturazione nei dati del cliente.

Premixed Primer:
Primer added by customer.

Standard Primer List:
Primer added by Microsynth. Select your sequencing primer from a list of 94 standard primers which are stored at Microsynth (primarily for plasmids).

Enclosed in Separate Tube:
Primer added by Microsynth. Customer ships primer and DNA in separate tubes to Microsynth.

Stored at Microsynth:
Primer added by Microsynth. Prerequisite for this option: You have already sent the primer to Microsynth in the past. Your primer will be stored for 6 months at Microsynth. The primer name will be automatically deleted from this list after 6 months or if the primer has been used up.

Custom Primer List:
Primer added by Microsynth. This option is an add-on to the option "Stored at Microsynth". If you know that a primer will be used frequently in future orders, send us a larger volume of this primer (at least 50μl) and add it to your custom primer list. We will then store it for an extended period (up to one year).

Order Now:
Primer added by Microsynth. Via this option you can order a new primer already during the order process without changing the order form. While your DNA is on the way to Microsynth, we will produce your sequencing primer and forward it to our sequencing lab.

Design at Microsynth:
Primer added by Microsynth. This option is similar to "Order Now". In addition to primer synthesis Microsynth performs also the primer design. You just have to provide us by email with a sequence of 50 - 200 bases.

Please mark this checkbox only if you know that your sample contains GC-rich sequences or hairpins.
If you work primarily with specific sequencing primers which you will use again and again over the coming months, Microsynth recommends you to make use of the “Custom Primer List”.

How does it work?
- If you have choosen the primer source „Order Now“ or „Enclosed in Separate Tube“, simply mark this box and the specific primer(s) of this order will be added to your „Custom Primer List“
- Or alternatively go to the “Options & Preferences” section and select “Review/Edit Custom Primer List” under “Primer Management”. Here you can add and delete specific sequencing primers.


Your overall benefits:
- Your specific primers will be stored at Microsynth
- When placing your next order, you can easily select the requested primer(s) from your “Custom Primer List” by one simple click.
- When working in a larger lab, you will have the possibility to share specific sequencing primers amongst your research group. For this purpose, however, you have to first create and define your group. This can be done within the “Options & Preferences” section when selecting “Create Group/Show Group Members” under “Group Management”.
Overview about Your Custom Primer List
NameSequenceRecieved AtExpires At

This pop up window gives you an overview about your current custom primer list. If you want to modify your custom primer list, please go to the “Options & Preferences” section of our webshop and select “Review/Edit Custom Primer List” under “Primer Management”.

The primer is not stored at Microsynth.
Possible reasons:
  1. The spelling of the primer name is wrong.
  2. The expiry date has exceeded and the primer was discarded.
  3. The primer has never been stored at Microsynth. Please check if you entered the correct primer source.
  4. The primer name is not yet saved in our sequencing data base because you plan to order the primer in the oligo webshop after this sequencing order. In this case, please choose the primer source “Design at Microsynth” or "Order Now".
cannot be offered within a punchout session, because the final price calculation depends on the samples you are turning in.
To order this service, you need to open the Microsynth webshop directly at the link below. This will end the current punchout session:
https://srvweb.microsynth.ch
or call us at:
+41 71 722 8333


Terms and Conditions
I accept the General Terms and Conditions of Sales of CARLO ERBA Reagents S.r.l. By pressing the Accept-button you will place your order for Microsynth AG products to CARLO ERBA Reagent s.r.l Microsynth’s Italian distributor.

Termini e Condizioni
Condizioni di Vendita della CARLO ERBA Reagents S.r.l. Premendo il bottone Accetto state formalmente facendo un ordine per prodotti Microsynth AG alla CARLO ERBA Reagent s.r.l distributore italiano della Microsynth AG.