Economy Run

Microsynth's standard sequencing service for plasmids and PCR products in 1.5 ml tubes.

Samples with prepaid green labels or non-prepaid blue labels can be registered here. For more details on sample preparation, please refer to our user guide.
Ecoli NightSeq®

Microsynth's innovative sequencing service for plasmids and PCR products in 1.5 ml tubes. Pick and transfer your E. coli colonies directly into our special tubes and receive your sequencing results the next day.

Samples with either prepaid red labels or non-prepaid red labels can be registered here. For more details on how to prepare your samples, please refer to our user guide.
Premium Run

Microsynth's customized Sanger sequencing service in 1.5 ml tubes, including comprehensive technical support and troubleshooting. This service is particularly suitable for customers who want the best possible results combined with superior service.

For more details on how to prepare your samples, please refer to our user guide.

Primer Walking - GMP Service

cGMP compliant Sanger sequencing service for the release testing of regulated biologics and for the validation of DNA-based diagnostic tests/devices for marketing authorization applications. Whether European or FDA-level submissions, Microsynth's quality system is designed to meet the most stringent quality requirements of regulatory authorities worldwide.

Reporting & Documentation includes sequencing data assembly, detailed description of the sequencing as well as extended GMP project reporting. Data is archived for 10 years (longer periods available on request).

Primer Walking - Standard Service

Microsynth's high quality sequencing service for whole plasmids and large PCR fragments. Uncomplicated and very fast service due to in-house primer production. Upon sequencing your sample, you will receive the electronic files of chromatograms as well as the assembled sequences (contigs) and consensus.

If a reference sequence is provided, Microsynth will verify your sequence and provide a variant report showing discrepancies compared to the reference sequence.
Economy Run

Microsynth's standard plate sequencing service for plasmids and PCR fragments. Samples can be registered here for the following service types:
Economy Run Plus Prepaid, Green Label Economy Run Prepaid, Green Label Economy Run Non-Prepaid, Blue Label
For more details on how to prepare your samples, please refer to our user guide.
Economy Run

Microsynth's standard plate sequencing service for plasmids and PCR fragments. Samples can be registered here for the following service types:
Economy Run Plus Prepaid, Green Label Economy Run Prepaid, Green Label Economy Run Non-Prepaid
For more details on how to prepare your samples, please refer to our user guide.

MORE THAN ONE PRIMER SOURCE PER PLATE?
Please use this order form only if you wish to specify more than one primer source on your 96-well plate.

Microsynth allows you to select your desired sequencing primer(s) from the following different primer sources:
  1. Premixed Primer
  2. Standard Primer List
  3. Custom Primer List
  4. Enclosed in Separate Tube
  5. Order Now Primer
  6. Designed at Microsynth
Economy Run

Microsynth's standard plate sequencing service for plasmids and PCR fragments. Samples can be registered here for the following service types:

Economy Run Non-Prepaid
FOR PREMIXED SAMPLES ONLY!

Please use this simplified order form only if you intend to send us samples that have already been pre-mixed with the appropriate sequencing primers in your laboratory.
Ecoli NightSeq®

Microsynth's innovative plate sequencing service for plasmids. Pick and transfer your E. coli colonies directly to our special plates and receive your sequencing results the next day.

Plates with either prepaid red labels or non-prepaid red labels can be registered here. For more details on how to prepare your samples, please refer to our user guide.
Ecoli NightSeq®

Microsynth's innovative plate sequencing service for plasmids. Pick and transfer your E. coli colonies directly to our special plates and receive your sequencing results the next day.

Plates with either prepaid red labels or non-prepaid red labels can be registered here. For more details on how to prepare your samples, please refer to our user guide.

MORE THAN ONE PRIMER SOURCE PER PLATE?
Please use this order form only if you wish to specify more than one primer source on your 96-well plate.
Microsynth allows you to select your desired sequencing primer(s) from the following different primer sources:

  1. Standard Primer List
  2. Custom Primer List
  3. Enclosed in Separate Tube
  4. Order Now Primer
  5. Designed at Microsynth
Ready-To-Load Run

Ideal for customers who wish to perform sample preparation, cycling, and purification in their own laboratory and outsource analysis on the capillary sequencer. Big dye 3.1 and 1.1 versions can be processed.
Order Upload

The Order Upload feature is a convenient tool for ordering our Economy Run Sanger sequencing plate services. It simplifies the process of ordering single or multiple plates by allowing you to upload automatically generated plate layout descriptions directly into the webshop.

For detailed instructions on the required file format, please download the file format description by clicking this link.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define sample names in column A (≤ 35 characters)
Step 3: Define corresponding primer names in column B (≤ 35 characters), Optional for primer source "premixed"
Step 4: Copy/paste sample and primer names into the foreseen window
Step 5: Press the "Vertical Import" button if you want to import your date in a vertical manner (A1, B1, C1, etc.) or "Horizontal Import" in case you prefer a horizontal arrangement of your data on the plate (A1, A2, A3, etc.)

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Optional: Put label numbers in column A.
If you use label numbers, then the colums below are shifted to the right by one column.
Step 3: Define sample names in column A (≤ 35 characters)
Step 4: Define corresponding primer names in column B (≤ 35 characters)
Optional for primer source "premixed"
Please see also Standard Primers or Customer Primer Management for possible primer names.
Step 5: Define corresponding primer sources in column C
Please use only one of the following terms: "Premixed", "Standard", "Enclosed", "Custom", "Order", "Design".
The primer source "Premixed" is only available for certain Dna Types.
Step 6: Define corresponding primer sequences in column D (Required only for Order Now primers)
Step 7: Copy/paste sample and primer names into the foreseen window
Step 8: Press the "Import" button

Example Example with label numbers

Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define sample names in column A (≤ 35 characters)
Step 3: Define corresponding primer names in column B (≤ 35 characters)
Optional for primer source "premixed"
Please see also Standard Primers or Customer Primer Management for possible primer names.
Step 4: Define corresponding primer sources in column C
Please use only one of the following terms: "Standard", "Enclosed", "Custom", "Order", "Design".
Step 5: Define corresponding primer sequences in column D (Required only for Order Now primers)
Step 6: Copy/paste sample and primer names into the foreseen window
Step 7: Press the "Import" button

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Optional: Put label numbers in column A.
Step 3: Define sample names in the next column (A or B).
Not more than 35 characters.
Step 4: Copy/paste sample names into the foreseen window
Step 5: Press the "Import" button

Example Example with label numbers

Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Step 1: Choose an oligo from your list of Favorites in the drop-down menu.
Step 2: Press "Add" to add the chosen oligo to your order.

Aliquoting Service

If you prefer to receive your oligonucleotides in small aliquots of defined amounts, you can make use of Microsynth’s aliquoting service for a small surcharge. Just select the desired amount (2 nmol or 5 nmol) and receive 10 aliquots of either amount as well as an additional tube with the leftover.

Please be aware that opting for the 2 nmol or 5 nmol default aliquoting service may not make sense for 1.0 µmol and 15 µmol synthesis scales since the majority of your oligonucleotide will be in the leftover tube. On request, however, we can also offer you a customized aliquoting service.

Technical Datasheet and Different Types of Certificate of Analysis (CoA)

On our technical datasheet, you find information regarding your ordered oligos like oligo name, oligo ID, nucleotide sequence, modifications, length, synthesis scale, molecular weight, melting point, the amount in OD, nmol and µg and the information with how much water you need to dissolve the dried oligo to obtain a 100 µM solution.

The technical datasheet does not meet your requirements?
For an extra charge, we do offer different types of CoAs for a higher level of documentation. Our standard CoA (Type 1) contains the following information:

Further to the standard CoA are the following types of CoA's available:

Type Description
1 Certificate of Analysis (complete)
2 CoA without declaration of oligonucleotide stability
3 CoA without MALDI-TOF
4 CoA without analytical HPLC
5 only Maldi-TOF spectra
6 only HPLC
7 CoA for ASO or PTO oligos (complete)

You need a customized CoA? Please get in contact with us.

How to Proceed:
Step 1: Use a standard Excel sheet
Step 2: Define oligo names in column A (≤ 35 characters)
Step 3: Define corresponding oligo sequences in column B
Step 4: Click on "Copy/paste"
Step 5: Copy/paste the oligo data from Excel into the foreseen window and press OK

Example


Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
Microsynth Oligonucleotides
Please be aware that Microsynth’s oligonucleotides are not sold through CARLO ERBA Reagents S.r.l. Oligonucleotide orders will be invoiced through Microsynth to the billing address in your customer data.

Microsynth Oligonucleotidi
Si prega di notare che gli oligonucleotidi di Microsynth non sono venduti tramite CARLO ERBA Reagents S.r.l. Gli ordini di oligonucleotidi verranno fatturati tramite Microsynth all'indirizzo di fatturazione nei dati del cliente.

Premixed Primer:
Primer added by customer.

Standard Primer List:
Primer added by Microsynth AG. Select your sequencing primer from a list of 94 standard primers which are stored at Microsynth AG (primarily for plasmids).

Enclosed in Separate Tube:
Primer added by Microsynth AG. Customer ships primer and DNA in separate tubes to Microsynth AG.

Stored at Microsynth AG:
Primer added by Microsynth AG. Prerequisite for this option: You have already sent the primer to Microsynth AG in the past. Your primer will be stored for 4 months at Microsynth AG. The primer name will be automatically deleted from this list after 4 months or if the primer has been used up.

Custom Primer List:
Primer added by Microsynth AG. This option is an add-on to the option "Stored at Microsynth AG". If you know that a primer will be used frequently in future orders, send us a larger volume of this primer (at least 50μl) and add it to your custom primer list. Your primer will be stored for 10 months at Microsynth AG. The primer name will be automatically deleted from this list after 10 months or if the primer has been used up.

Order Now:
Primer added by Microsynth AG. Via this option you can order a new sequencing primer during the order process. While your sample is on the way to Microsynth AG, we synthesize this primer, and it will be available for sequencing.

Design at Microsynth AG:
Primer added by Microsynth AG. This option is similar to "Order Now". In addition to primer synthesis Microsynth AG performs also the primer design. You just have to provide us by email with a sequence of 50 - 200 bases.

Please mark this checkbox only if you know that your sample contains GC-rich sequences or hairpins.
If you work primarily with specific sequencing primers which you will use again and again over the coming months, Microsynth AG recommends you to make use of the “Custom Primer List”.

How does it work?
- If you have choosen the primer source „Order Now“ or „Enclosed in Separate Tube“, simply mark this box and the specific primer(s) of this order will be added to your „Custom Primer List“
- Or alternatively go to the “Options & Preferences” section and select “Review/Edit Custom Primer List” under “Primer Management”. Here you can add and delete specific sequencing primers.


Your overall benefits:
- Your specific primers will be stored at Microsynth AG
- When placing your next order, you can easily select the requested primer(s) from your “Custom Primer List” by one simple click.
- When working in a larger lab, you will have the possibility to share specific sequencing primers amongst your research group. For this purpose, however, you have to first create and define your group. This can be done within the “Options & Preferences” section when selecting “Create Group/Show Group Members” under “Group Management”.
Full PlasmidSeq is suitable for sequencing constructs ranging from 0.5 kB to 50 kB, with the ideal size for plasmids being 2.5 to 25 kB and for PCR fragments being 1 to 25 kB.
If you have larger constructs to sequence, please feel free to contact us.
To ensure good sequencing results please aim for a concentration of 20 ng/ul (measured by fluorescence, not UV 260).
Please note that with the current approach, the ends of PCR fragments may not be fully resolved, resulting in a loss of approximately 10-50 bases at each end.
Overview about Your Custom Primer List
NameSequenceReceived AtExpires At

This pop up window gives you an overview about your current custom primer list. If you want to modify your custom primer list, please go to the “Options & Preferences” section of our webshop and select “Review/Edit Custom Primer List” under “Primer Management”.

cannot be offered within a punchout session, because the final price calculation depends on the samples you are turning in.
To order this service, you need to open the Microsynth AG webshop directly at the link below. This will end the current punchout session:
Standard webshop
or call us at:
+41 71 722 8333


Terms and Conditions
I accept the General Terms and Conditions of Sales of CARLO ERBA Reagents S.r.l. By pressing the Accept-button you will place your order for Microsynth AG products to CARLO ERBA Reagent s.r.l Microsynth’s Italian distributor.

Termini e Condizioni
Condizioni di Vendita della CARLO ERBA Reagents S.r.l. Premendo il bottone Accetto state formalmente facendo un ordine per prodotti Microsynth AG alla CARLO ERBA Reagent s.r.l distributore italiano della Microsynth AG.

The Microsynth Offer Number:
The Microsynth offer number is no longer needed.
Specific "QTN-..." offers for oligo and Sanger label orders can be found in the Quotes list - from where they must be released.
The link to the quotes list is also located in the header area of this webshop.
The prices of the "SQ-..." offers will be automatically applied to your account.

Either way, the displayed prices are binding.

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