Aliquoting Service
If you prefer to receive your oligonucleotides in small aliquots of defined amounts, you can make use of Microsynth’s aliquoting service for a small surcharge. Just select the desired amount (2 nmol or 5 nmol) and receive 10 aliquots of either amount as well as an additional tube with the leftover.
Please be aware that opting for the 2 nmol or 5 nmol default aliquoting service may not make sense for 1.0 µmol and 15 µmol synthesis scales since the majority of your oligonucleotide will be in the leftover tube. On request, however, we can also offer you a customized aliquoting service.
When you need oligonucleotides with degenerated bases, Microsynth AG can offer you the following 3 product options:
*Bases are automatically mixed by synthesizer.
| Product | Applications | How to order |
|---|---|---|
| Degenerated bases (IUB Hand Mixed Synthesis Reagents) | NGS, barcoding and mutagenesis applications where equal distribution of degenerate bases is critical. | Requires a specific quote |
| Degenerated Bases (Custom Hand Mixed Synthesis Reagents) | ||
| Degenerated Bases (Standard IUB Wobbles)* | All other applications where equal distribution is less important or the sequence space is too big to be covered with one standard synthesis i.e. some SELEX applications | Directly via webshop |
CC Email Address:
Enter addtional email addresses to receive a copy of the test results.
Separte addresses with commas.
How to Proceed:
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
| Step 1: | Use a standard Excel sheet |
| Step 2: | Define sample names in column A (≤ 35 characters) |
| Step 3: | Define corresponding primer names in column B (≤ 35 characters). Optional for primer source Premixed |
| Step 4: | Copy/paste sample and primer names into the foreseen window |
| Step 5: | Press the "Vertical Import" button if you want to import your date in a vertical manner (A1, B1, C1, etc.) or "Horizontal Import" in case you prefer a horizontal arrangement of your data on the plate (A1, A2, A3, etc.) |
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
| Step 1: | Use a standard Excel sheet |
| Step 2: |
Optional:
Put label numbers in column A. If you use label numbers, then the colums below are shifted to the right by one column. |
| Step 3: | Define sample names in column A (≤ 35 characters) |
| Step 4: |
Define corresponding primer names in column B (≤ 35 characters). Optional for primer source Premixed Please see also Standard Primers or Customer Primer Management for possible primer names. |
| Step 5: |
Define corresponding primer sources in column C Please use only one of the following terms: Premixed, Standard, Enclosed, Custom, Order, Design. The primer source "Premixed" is only available for certain Dna Types. |
| Step 6: | Define corresponding primer sequences in column D (Required only for Order Now primers) |
| Step 7: | Copy/paste sample and primer names into the foreseen window |
| Step 8: | Press the "Import" button |
| Example | Example with label numbers |
 |
 |
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
| Step 1: | Use a standard Excel sheet |
| Step 2: | Define sample names in column A (≤ 35 characters) |
| Step 3: |
Define corresponding primer names in column B (≤ 35 characters). Optional for primer source Premixed Please see also Standard Primers or Customer Primer Management for possible primer names. |
| Step 4: |
Define corresponding primer sources in column C Please use only one of the following terms: Standard, Enclosed, Custom, Order, Design. |
| Step 5: | Define corresponding primer sequences in column D (Required only for Order Now primers) |
| Step 6: | Copy/paste sample and primer names into the foreseen window |
| Step 7: | Press the "Import" button |
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
| Step 1: | Use a standard Excel sheet |
| Step 2: | Optional: Put label numbers in column A. |
| Step 3: |
Define sample names in the next column (A or B). Not more than 35 characters. |
| Step 4: | Copy/paste sample names into the foreseen window |
| Step 5: | Press the "Import" button |
| Example | Example with label numbers |
 |
 |
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
How to Proceed:
| Step 1: | Choose an oligo from your list of Favorites in the drop-down menu. |
| Step 2: | Press "Add" to add the chosen oligo to your order. |
DNA Oligo Scales
Please find more info on our web pages Scale Info.
RNA Oligo Scales
Please find more info on our web pages Scale Info.
Oligo Purification
Please find more info on our web pages Purification Info.
Technical Datasheet and Different Types of Certificate of Analysis (CoA)
On our technical datasheet, you find information regarding your ordered oligos like oligo name, oligo ID, nucleotide sequence, modifications, length, synthesis scale, molecular weight, melting point, the amount in OD, nmol and µg and the information with how much water you need to dissolve the dried oligo to obtain a 100 µM solution.
The technical datasheet does not meet your requirements?
For an extra charge, we do offer different types of CoAs for a higher level of documentation.
Our standard CoA (Type 1) contains the following information:
- Appearance test by means of visual inspection
- Solubility test by means of solubilization in buffer prior to lyophilization
- Information about re-suspension of your oligo and the stability (shelf life at different storage conditions)
- Identity test by means of MALDI-TOF including the chromatogram
- Purity test by means of analytical HPLC including the chromatogram (if secondary structure is too strong, we do not perform this analysis)
- Purity test by means of analytical PAGE
- Proof checked and signed by our head of oligonucleotide department or the delegated representative
Further to the standard CoA are the following types of CoA's available:
| Type | Description |
|---|---|
| 1 | Certificate of Analysis (complete) |
| 2 | CoA without declaration of oligonucleotide stability |
| 3 | CoA without MALDI-TOF |
| 4 | CoA without analytical HPLC |
| 5 | only Maldi-TOF spectra |
| 6 | only HPLC |
| 7 | CoA for ASO or PTO oligos (complete) |
You need a customized CoA? Please get in contact with us.
How to Proceed:
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
| Step 1: | Use a standard Excel sheet |
| Step 2: | Define oligo names in column A (≤ 35 characters) |
| Step 3: | Define corresponding oligo sequences in column B |
| Step 4: | Click on "Copy/paste" |
| Step 5: | Copy/paste the oligo data from Excel into the foreseen window and press OK |
Example
Important Remark
Don’t use : ; , " \ / * ? < > | # Tabulator.
Microsynth OligonucleotidesPlease be aware that Microsynth’s oligonucleotides are not sold through CARLO ERBA Reagents S.r.l. Oligonucleotide orders will be invoiced through Microsynth to the billing address in your customer data.
Microsynth Oligonucleotidi
Si prega di notare che gli oligonucleotidi di Microsynth non sono venduti tramite CARLO ERBA Reagents S.r.l. Gli ordini di oligonucleotidi verranno fatturati tramite Microsynth all'indirizzo di fatturazione nei dati del cliente.
Premixed Primer
Primer added by customer.
Standard Primer List
Primer added by Microsynth AG. Select your sequencing primer from a list of 94 standard primers which are stored at Microsynth AG (primarily for plasmids).
Enclosed in Separate Tube
Primer added by Microsynth AG. Customer ships primer and DNA in separate tubes to Microsynth AG.
Custom Primer List
Primer added by Microsynth AG.
If you know that a primer will be used frequently in future orders, send us a larger volume of this primer (at least 50μl) and add it to your custom primer list.
Your primer will be stored for 10 months at Microsynth AG.
The primer name will be automatically deleted from this list after 10 months or if the primer has been used up.
Order Now
Primer added by Microsynth AG. Via this option you can order a new sequencing primer during the order process.
While your sample is on the way to Microsynth AG, we synthesize this primer, and it will be available for sequencing.
The primer will also be added to your custom primer list for future use.
Design at Microsynth AG
Primer added by Microsynth AG. This option is similar to "Order Now".
In addition to primer synthesis Microsynth AG performs also the primer design.
You just have to provide us with a sequence of 50 - 200 bases by email.
Max length 36 chars.
Max length 100 chars.
Max length 150 chars.
Max length 250 chars.
Please mark this checkbox only if you know that your sample contains GC-rich sequences or hairpins.
Full PlasmidSeq is suitable for sequencing constructs ranging from 0.5 kB to 50 kB,
with the ideal size for plasmids being 2.5 to 25 kB and for PCR fragments being 1 to 25 kB.
If you have larger constructs to sequence, please feel free to contact us.
If you have larger constructs to sequence, please feel free to contact us.
To ensure good sequencing results please aim for a concentration of 20 ng/ul (measured by fluorescence, not UV 260).
Please note that with the current approach, the ends of PCR fragments may not be fully resolved,
resulting in a loss of approximately 10-50 bases at each end.
Overview about Your Custom Primer List
This pop up window gives you an overview about your current custom primer list. If you want to modify your custom primer list, leave this order form and go to Primer Management.
cannot be offered within a punchout session,
because the final price calculation depends on the samples you are turning in.
To order this service, you need to open the Microsynth AG webshop directly at the link below. This will end the current punchout session:
Standard webshop
or call us at:
+41 71 722 8333
Terms and Conditions
I accept the General Terms and Conditions of Sales of CARLO ERBA Reagents S.r.l. By pressing the Accept-button you will place your order for Microsynth AG products to CARLO ERBA Reagent s.r.l Microsynth’s Italian distributor.
Termini e Condizioni
Condizioni di Vendita della CARLO ERBA Reagents S.r.l. Premendo il bottone Accetto state formalmente facendo un ordine per prodotti Microsynth AG alla CARLO ERBA Reagent s.r.l distributore italiano della Microsynth AG.
The Microsynth Offer Number:
The Microsynth offer number is no longer needed.
Specific "QTN-..." offers for oligo and Sanger label orders can be found in the Quotes list - from where they must be released.
The link to the quotes list is also located in the header area of this webshop.
The prices of the "SQ-..." offers will be automatically applied to your account.
Either way, the displayed prices are binding.
The Microsynth offer number is no longer needed.
Specific "QTN-..." offers for oligo and Sanger label orders can be found in the Quotes list - from where they must be released.
The link to the quotes list is also located in the header area of this webshop.
The prices of the "SQ-..." offers will be automatically applied to your account.
Either way, the displayed prices are binding.